Nucleic Acid Amplification Technology from Olink Bioscience

Circle-to-circle amplification

Circle-to-circle amplification (C2CA) enables high precision strand-specific amplification of circularized DNA molecules. C2CA is not product inhibited, and can therefore yield 100-fold higher concentrations of monomer products than PCR. C2CA can be used for multiplexed genotyping of polymorphic loci and for quantitative DNA analysis.

How does circle-to-circle amplification work?

Tandem repeated complements of DNA circles (e.g. a circular Padlock probe) are generated by rolling circle replication, and converted to monomer circles of opposite polarity to that of the starting material. These circles are then subjected to one more round of rolling circle replication and circularization, and the process can be further repeated. The method can be directed to produce single stranded circular or linear monomers, or linear concatemers of the desired polarity. Each generation of the amplification process proceeds in a linear fashion, ensuring precise quantification. The procedure is suitable for parallel amplification of large numbers of DNA circles, because the few cycles and the robust reaction mechanism preserve the proportion of amplified molecules.



For peer-reviewed information about circle-to-circle amplification, read Dahl et al., PNAS (2004) 13:4548-4553.