Technology / Publications
Publications on Olink Bioscience Technologies
Protein Detection using PLA
In situ PLA using Duolink
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Bougatef F, Quemener C, Kellouche S, Naïmi B, Podgorniak MP, Millot G, Gabison EE, Calvo F, Dosquet C, Lebbé C, Menashi S, Mourah S. EMMPRIN promotes angiogenesis through hypoxia-inducible factor-2alpha-mediated regulation of soluble VEGF isoforms and their receptor VEGFR-2. Blood. 2009 Dec 24;114(27):5547-56. Epub 2009 Oct 16.
Matic I, Schimmel J, Hendriks IA, van Santen MA, van de Rijke F, van Dam H, Gnad F, Mann M, Vertegaal AC. Site-Specific Identification of SUMO-2 Targets in Cells Reveals an Inverted SUMOylation Motif and a Hydrophobic Cluster SUMOylation Motif. Mol Cell. 2010 Aug 27;39(4):641-652.
Winkler EA, Bell RD, Zlokovic BV. Pericyte-specific expression of PDGF beta receptor in mouse models with normal and deficient PDGF beta receptor signaling. Mol Neurodegener. 2010 Aug 25;5(1):32.
Olsen BB, Issinger OG, Guerra B. Regulation of DNA-dependent protein kinase by protein kinase CK2 in human glioblastoma cells. Oncogene. 2010 Aug 16.
M Aubele, M Spears, N Ludyga, H Braselmann, A Feuchtinger, KJ Taylor, K Lindner, G Auer, K Stering, H Höfler,M Schmitt and JMS Bartlett. In situ quantification of HER2–protein tyrosine kinase 6 (PTK6) protein–protein complexes in paraffin sections from breast cancer tissues. British Journal of Cancer 10 August, 2010.
Addolorata Pisconti, D.D.W. Cornelison, Hugo C. Olguín, Tiffany L. Antwine, and Bradley B. Olwin. Syndecan-3 and Notch cooperate in regulating adult myogenesis. JCB, August 9, 2010
Kaori Saito , Nobuhiro Nakamu, Yusuke Ito, Kazuyuki Hoshij, Masahiro Esaki Esaki, Boqiang Zhao and Shigehisa Hirose. Identification of zebrafish FXYD11a protein that is highly expressed in iontransporting epithelium of the gill and skin and its possible role in ion homeostasis. Frontiers in Aquatic Physiology, 07 Aug 2010.
Angermeier M, Eckardt-Schupp F, Moertl S. A novel function of Ubc13 in TNFR1 receptor activation. Cell Signal. 2010 Sep;22(9):1388-96.
Hsu KH, Tsai HW, Lin PW, Hsu YS, Shan YS, Lu PJ. Clinical implication and mitotic effect of CD44 cleavage in relation to osteopontin/CD44 interaction and dysregulated cell cycle protein in gastrointestinal stromal tumor. Ann Surg Oncol. 2010 Aug;17(8):2199-212.
Raizer JJ, Grimm S, Chamberlain MC, Nicholas MK, Chandler JP, Muro K, Dubner S, Rademaker AW, Renfrow J, Bredel M. A phase 2 trial of single-agent bevacizumab given in an every-3-week schedule for patients with recurrent high-grade gliomas. Cancer. 2010 Jul 27.
Nakahara KS, Shimada R, Choi SH, Yamamoto H, Shao J, Uyeda I. Involvement of the P1 cistron in overcoming eIF4E-mediated recessive resistance against Clover yellow vein virus in pea. Mol Plant Microbe Interact. 2010 Jul 23.
Nakatsukasa M, Kawasaki S, Yamasaki K, Fukuoka H, Matsuda A, Tsujikawa M, Tanioka H, Nagata-Takaoka M, Hamuro J, Kinoshita S. Tumor-Associated Calcium Signal Transducer 2 Is Required for the Proper Subcellular Localization of Claudin 1 and 7. Implications in the Pathogenesis of Gelatinous Drop-Like Corneal Dystrophy. Am J Pathol. 2010 Jul 22.
Lasserre R, Charrin S, Cuche C, Danckaert A, Thoulouze MI, de Chaumont F, Duong T, Perrault N, Varin-Blank N, Olivo-Marin JC, Etienne-Manneville S, Arpin M, Di Bartolo V, Alcover A. Ezrin tunes T-cell activation by controlling Dlg1 and microtubule positioning at the immunological synapse. EMBO J. 2010 Jul 21;29(14):2301-14.
Sarparanta J, Blandin G, Charton K, Vihola A, Marchand S, Milic A, Hackman P, Ehler E, Richard I, Udd B. Interactions with M-band titin and calpain 3 link myospryn (CMYA5) to tibial and limb-girdle muscular dystrophies. J Biol Chem. 2010 Jul 15.
Schneider G, Nieznanski K, Jozwiak J, Slomnicki LP, Redowicz MJ, Filipek A. Tubulin binding protein, CacyBP/SIP, induces actin polymerization and may link actin and tubulin cytoskeletons. Biochim Biophys Acta. 2010 Jul 14.
Ling SC, Albuquerque CP, Han JS, Lagier-Tourenne C, Tokunaga S, Zhou H, Cleveland DW. ALS-associated mutations in TDP-43 increase its stability and promote TDP-43 complexes with FUS/TLS. Proc Natl Acad Sci U S A. 2010 Jul 12.
Mengistu M, Brotzman H, Ghadiali S, Lowe-Krentz L. Fluid shear stress-induced JNK activity leads to actin remodeling for cell alignment. J Cell Physiol. 2010 Jul 12.
Chen YJ, Chen YH, Chow LP, Tsai YH, Chen PH, Huang CY, Chen WT, Hwang LH. Heat shock protein 72 is associated with the hepatitis C virus replicase complex and enhances viral RNA replication. J Biol Chem. 2010 Jul 2.
Akbari M, Solvang-Garten K, Hanssen-Bauer A, Lieske NV, Pettersen HS, Pettersen GK, Wilson DM 3rd, Krokan HE, Otterlei M. Direct interaction between XRCC1 and UNG2 facilitates rapid repair of uracil in DNA by XRCC1 complexes. DNA Repair (Amst). 2010 Jul 1;9(7):785-95.
Edin S, Oruganti SR, Grundström C, Grundström T. Interaction of calmodulin with Bcl10 modulates NF-kappaB activation. Mol Immunol. 2010 Jul;47(11-12):2057-64.
Hervouet E, Lalier L, Debien E, Cheray M, Geairon A, Rogniaux H, Loussouarn D, Martin SA, Vallette FM, Cartron PF. Disruption of Dnmt1/PCNA/UHRF1 Interactions Promotes Tumorigenesis from Human and Mice Glial Cells. PLoS ONE, 2010 Jun 29;5(6):e11333.
Yang H, Hreggvidsdottir HS, Palmblad K, Wang H, Ochani M, Li J, Lu B, Chavan S, Rosas-Ballina M, Al-Abed Y, Akira S, Bierhaus A, Erlandsson-Harris H, Andersson U, Tracey KJ. A critical cysteine is required for HMGB1 binding to Toll-like receptor 4 and activation of macrophage cytokine release. Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11942-7.
Kantidakis T, Ramsbottom BA, Birch JL, Dowding SN, White RJ. mTOR associates with TFIIIC, is found at tRNA and 5S rRNA genes, and targets their repressor Maf1. Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11823-8.
Larsen JV, Hansen M, Møller B, Madsen P, Scheller J, Nielsen M, Petersen CM. Sortilin facilitates signaling of CNTF and related helical type-1 cytokines targeting the gp130/LIFR{beta} heterodimer. Mol Cell Biol. 2010 Jun 28. [Epub ahead of print]
Chen JS, Hsu YM, Chen CC, Chen LL, Lee CC, Huang TS. Secreted heat shock protein 90α induces colorectal cancer cell invasion through CD91/LRP-1 and NF-κB-mediated integrin αV expression. J Biol Chem. 2010 Jun 17. [Epub ahead of print]
Hsu KH, Tsai HW, Lin PW, Hsu YS, Shan YS, Lu PJ. Osteopontin Expression is an Independent Adverse Prognostic Factor in Resectable Gastrointestinal Stromal Tumor and its Interaction with CD44 Promotes Tumor Proliferation. Ann Surg Oncol. 2010 Jun 12. [Epub ahead of print]
Brobeil A, Graf M, Oeschger S, Steger K, Wimmer M. PTPIP51—a myeloid lineage specific protein interacts with PTP1B in neutrophil granulocytes. Blood Cells Mol Dis. 2010 Jun 1. [Epub ahead of print]
Johansson H, Vizlin-Hodzic D, Simonsson T, Simonsson S. Translationally controlled tumor protein interacts with nucleophosmin during mitosis in ES cells. Cell Cycle. 2010 Jun 1;9(11):2160-69.
Baan B, Pardali E, Ten Dijke P, van Dam H. In situ proximity ligation detection of cJun/AP-1 dimers reveals increased levels of cJun/Fra1 complexes in aggressive breast cancer cell lines in vitro and in vivo. Mol Cell Proteomics. 2010 May 28. [Epub ahead of print]
Nakakuki T, Birtwistle MR, Saeki Y, Yumoto N, Ide K, Nagashima T, Brusch L, Ogunnaike BA, Okada-Hatakeyama M, Kholodenko BN. Ligand-Specific c-Fos Expression Emerges from the Spatiotemporal Control of ErbB Network Dynamics. Cell. 2010 May 28;141(5):884-96.
Venkatesan JK, Natarajan S, Schwarz K, Mayer SI, Alpadi K, Magupalli VG, Sung CH, Schmitz F. Nicotinamide adenine dinucleotide-dependent binding of the neuronal Ca2+ sensor protein GCAP2 to photoreceptor synaptic ribbons. J Neurosci. 2010 May 12;30(19):6559-76.
Bonagura TW, Aberdeen GW, Babischkin JS, Koos RD, Pepe GJ, Albrecht ED. Divergent regulation of angiopoietin-1 and -2, Tie-2, and thrombospondin-1 expression by estrogen in the baboon endometrium. Mol Reprod Dev. 2010 May;77(5):430-8.
Baima ET, Guzova JA, Mathialagan S, Nagiec EE, Hardy MM, Song LR, Bonar SL, Weinberg RA, Selness SR, Woodard SS, Chrencik J, Hood WF, Schindler JF, Kishore N, Mbalaviele G. Novel insights into the cellular mechanisms of the anti-inflammatory effects of NF-kappaB essential modulator binding domain peptides. J Biol Chem. 2010 Apr 30;285(18):13498-506.
Nilsson I, Bahram F, Li X, Gualandi L, Koch S, Jarvius M, Söderberg O, Anisimov A, Kholová I, Pytowski B, Baldwin M, Ylä-Herttuala S, Alitalo K, Kreuger J, Claesson-Welsh L. VEGF receptor 2/-3 heterodimers detected in situ by proximity ligation on angiogenic sprouts.EMBO J. 2010 Apr 21;29(8):1377-88.
Shafey D, Boyer JG, Bhanot K, Kothary R. Identification of Novel Interacting Protein Partners of SMN Using Tandem Affinity Purification. J Proteome Res. 2010 Apr 5;9(4):1659-69.
Vetterkind S, Lee E, Sundberg E, Poythress RH, Tao TC, Preuss U, Morgan KG. Par-4: a new activator of myosin phosphatase. Mol Biol Cell. 2010 Apr;21(7):1214-24.
Gurumurthy RK, Mäurer AP, Machuy N, Hess S, Pleissner KP, Schuchhardt J, Rudel T, Meyer TF.A loss-of-function screen reveals Ras- and Raf-independent MEK-ERK signaling during Chlamydia trachomatis infection. Sci Signal. 2010 Mar 16;3(113):ra21.
Wilhelm MT, Rufini A, Wetzel MK, Tsuchihara K, Inoue S, Tomasini R, Itie-Youten A, Wakeham A, Arsenian-Henriksson M, Melino G, Kaplan DR, Miller FD, Mak TW. Isoform-specific p73 knockout mice reveal a novel role for {Delta}Np73 in the DNA damage response pathway. Genes Dev. 2010 Mar 15;24(6):549-60.
Farrar D, Rai S, Chernukhin I, Jagodic M, Ito Y, Yammine S, Ohlsson R, Murrell A, Klenova E. Mutational analysis of the poly(ADP-ribosyl)ation sites of the transcription factor CTCF provides an insight into the mechanism of its regulation by poly(ADP-ribosyl)ation. Mol Cell Biol. 2010 Mar;30(5):1199-216.
Chen TT, Luque A, Lee S, Anderson SM, Segura T, Iruela-Arispe ML. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol. 2010 Feb 22;188(4):595-609.
Sehat B, Tofigh A, Lin Y, Trocmé E, Liljedahl U, Lagergren J, Larsson O. SUMOylation Mediates the Nuclear Translocation and Signaling of the IGF-1 Receptor. Sci Signal. 2010 Feb 9;3(108):ra10.
Conze T, Carvalho AS, Landegren U, Almeida R, Reis CA, David L, Söderberg O. MUC2 mucin is a major carrier of the cancer-associated sialyl-Tn antigen in intestinal metaplasia and gastric carcinomas. Glycobiology. 2010 Feb;20(2):199-206.
Gajadhar A, Guha A. A proximity ligation assay using transiently transfected, epitope-tagged proteins: application for in situ detection of dimerized receptor tyrosine kinases. Biotechniques. 2010 Feb;48(2):145-52.
Sakane A, Honda K, Sasaki T. Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2. Mol Cell Biol. 2010 Feb;30(4):1077-87.
Liu B, Larsson L, Caballero A, Hao X, Oling D, Grantham J, Nyström T. The polarisome is required for segregation and retrograde transport of protein aggregates. Cell. 2010 Jan 22;140(2):257-67.
Frei R, Steinle J, Birchler T, Loeliger S, Roduit C, Steinhoff D, Seibl R, Büchner K, Seger R, Reith W, Lauener RP. MHC class II molecules enhance Toll-like receptor mediated innate immune responses. PLoS ONE, 2010 Jan 20;5(1):e8808.
Kii I, Nishiyama T, Li M, Matsumoto K, Saito M, Amizuka N, Kudo A.Incorporation of tenascin-C into the extracellular matrix by periostin underlies an extracellular meshwork architecture. J Biol Chem. 2010 Jan 15;285(3):2028-39.
Leuchowius KJ, Jarvius M, Wickström M, Rickardson L, Landegren U, Larsson R, Söderberg O, Fryknäs M, Jarvius J. High content screening for inhibitors of protein interactions and post-translational modifications in primary cells by proximity ligation. Mol Cell Proteomics. 2010 Jan;9(1):178-83.
Zieba A, Wählby C, Hjelm F, Jordan L, Berg J, Landegren U, Pardali K.. Bright-field microscopy visualization of proteins and protein complexes by in situ proximity ligation with peroxidase detection. Clin Chem. 2010 Jan;56(1):99-110.
Robinson SD, Reynolds LE, Kostourou V, Reynolds AR, da Silva RG, Tavora B, Baker M, Marshall JF, Hodivala-Dilke KM. Alphav beta3 integrin limits the contribution of neuropilin-1 to vascular endothelial growth factor-induced angiogenesis. J Biol Chem. 2009 Dec 4;284(49):33966-81.
Svenningsen P, Uhrenholt TR, Palarasah Y, Skjødt K, Jensen BL, Skøtt O. Prostasin-dependent activation of epithelial Na+ channels by low plasmin concentrations. Am J Physiol Regul Integr Comp Physiol. 2009 Dec;297(6):R1733-41.
Hasumi Y, Baba M, Ajima R, Hasumi H, Valera VA, Klein ME, Haines DC, Merino MJ, Hong SB, Yamaguchi TP, Schmidt LS, Linehan WM. Homozygous loss of BHD causes early embryonic lethality and kidney tumor development with activation of mTORC1 and mTORC2. Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18722-7.
Smith R, Bacos K, Fedele V, Soulet D, Walz HA, Obermüller S, Lindqvist A, Björkqvist M, Klein P, Onnerfjord P, Brundin P, Mulder H, Li JY. Mutant huntingtin interacts with {beta}-tubulin and disrupts vesicular transport and insulin secretion. Hum Mol Genet. 2009 Oct 15;18(20):3942-54.
Yadavilli S, Mayo LD, Higgins M, Lain S, Hegde V, Deutsch WA.Ribosomal protein S3: A multi-functional protein that interacts with both p53 and MDM2 through its KH domain.DNA Repair (Amst). 2009 Oct 2;8(10):1215-24.
Hervouet E, Vallette FM, Cartron PF. Dnmt3/transcription factor interactions as crucial players in targeted DNA methylation. Epigenetics. 2009 Oct;4(7):487-99.
Koos B, Paulsson J, Jarvius M, Sanchez BC, Wrede B, Mertsch S, Jeibmann A, Kruse A, Peters O, Wolff JE, Galla HJ, Söderberg O, Paulus W, Ostman A, Hasselblatt M. Platelet-Derived Growth Factor Receptor Expression and Activation in Choroid Plexus Tumors. Am J Pathol. 2009 Oct;175(4):1631-7.
Leuchowius KJ, Weibrecht I, Landegren U, Gedda L, Söderberg O. Flow cytometric in situ proximity ligation analyses of protein interactions and post-translational modification of the epidermal growth factor receptor family. Cytometry A. 2009 Oct;75(10):833-9.
Massinen S, Tammimies K, Tapia-Páez I, Matsson H, Hokkanen ME, Söderberg O, Landegren U, Castrén E, Gustafsson JA, Treuter E, Kere J. Functional interaction of DYX1C1 with estrogen receptors suggests involvement of hormonal pathways in dyslexia. Hum Mol Genet. 2009 Aug 1;18(15):2802-12.
Yadav AK, Renfrow JJ, Scholtens DM, Xie H, Duran GE, Bredel C, Vogel H, Chandler JP, Chakravarti A, Robe PA, Das S, Scheck AC, Kessler JA, Soares MB, Sikic BI, Harsh GR, Bredel M. Monosomy of chromosome 10 associated with dysregulation of epidermal growth factor signaling in glioblastomas. JAMA. 2009 Jul 15;302(3):276-89.
Tan SH, Pal M, Tan MJ, Wong MH, Tam FU, Teo JW, Chong HC, Tan CK, Goh YY, Tang MB, Cheung PC, Tan NS. Regulation of cell proliferation and migration by TAK1 via transcriptional control of von hippel-lindau tumor suppressor. J Biol Chem. 2009 Jul 3;284(27):18047-58.
Laane E, Tamm KP, Buentke E, Ito K, Kharaziha P, Oscarsson J, Corcoran M, Björklund AC, Hultenby K, Lundin J, Heyman M, Söderhäll S, Mazur J, Porwit A, Pandolfi PP, Zhivotovsky B, Panaretakis T, Grandér D. Cell death induced by dexamethasone in lymfoid leukemia is mediated through initiation of autophagy. Cell Death Differ. 2009 Jul;16(7):1018-29.
Tuomi S, Mai A, Nevo J, Laine JO, Vilkki V, Ohman TJ, Gahmberg CG, Parker PJ, Ivaska J. PKCepsilon regulation of an alpha5 integrin-ZO-1 complex controls lamellae formation in migrating cancer cells. Sci Signal. 2009 Jun 30;2(77):ra32.
Mellberg S, Dimberg A, Bahram F, Hayashi M, Rennel E, Ameur A, Westholm JO, Larsson E, Lindahl P, Cross MJ, Claesson-Welsh L. Transcriptional profiling reveals a critical role for tyrosine phosphatase VE-PTP in regulation of VEGFR2 activity and endothelial cell morphogenesis. FASEB J. 2009 May;23(5):1490-502.
Yamazaki T, Yoshimatsu Y, Morishita Y, Miyazono K, Watabe T. COUP-TFII regulates the functions of Prox1 in lymphatic endothelial cells through direct interaction. Genes Cells. 2009 Mar;14(3):425-34.
Matsumoto T, Schiller P, Dieterich LC, Bahram F, Iribe Y, Hellman U, Wikner C, Chan G, Claesson-Welsh L, Dimberg A. Ninein is expressed in the cytoplasm of angiogenic tip-cells and regulates tubular morphogenesis of endothelial cells. Arterioscler Thromb Vasc Biol. 2008 Dec;28(12):2123-30.
Greenberg JI, Shields DJ, Barillas SG, Acevedo LM, Murphy E, Huang J, Scheppke L, Stockmann C, Johnson RS, Angle N, Cheresh DA. A role for VEGF as a negative regulator of pericyte function and vessel maturation. Nature. 2008 Dec 11;456(7223):809-13.
Vermeer MH, van Doorn R, Dijkman R, Mao X, Whittaker S, van Voorst Vader PC, Gerritsen MJ, Geerts ML, Gellrich S, Söderberg O, Leuchowius KJ, Landegren U, Out-Luiting JJ, Knijnenburg J, Ijszenga M, Szuhai K, Willemze R, Tensen CP. Novel and highly recurrent chromosomal alterations in Sézary syndrome.Cancer Res. 2008 Apr 15;68(8):2689-98.
Szado T., Vanderheyden V., Parys JB., De Smedt H., Rietdorf K., Kotelevets L., Chastre E., Khan F., Landegren U., Söderberg O., Bootman MD., Roderick HL. Phosphorylation of inositol 1,4,5-trisphosphate receptors by protein kinase B/Akt inhibits Ca2+ release and apoptosis. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2427-32.
Additional related publications: Paulsson J, Lindh MB, Jarvius M, Puputti M, Nistér M, Nupponen NN, Paulus W, Söderberg O, Dresemann G, von Deimling A, Joensuu H, Ostman A, Hasselblatt M. Prognostic but not predictive role of platelet-derived growth factor receptors in patients with recurrent glioblastoma. Int J Cancer. 2010 Jun 29. [Epub ahead of print]
Weibrecht I, Leuchowius KJ, Clausson CM, Conze T, Jarvius M, Howell WM, Kamali-Moghaddam M, Söderberg O. Proximity ligation assays: a recent addition to the proteomics toolbox. Expert Rev Proteomics. 2010 Jun;7(3):401-9.
Schlingemann J, Leijon M, Yacoub A, Schlingemann H, Zohari S, Matyi-Tóth A, Kiss I, Holmquist G, Nordengrahn A, Landegren U, Ekström B, Belák S. Novel means of viral antigen identification: improved detection of avian influenza viruses by proximity ligation. J Virol Methods. 2010 Jan;163(1):116-22.
Spears M, Bartlett JM. Human epidermal growth factor receptor dimerization analysis in breast cancer diagnosis: potential for improving testing accuracy and treatment selection. Mol Diagn Ther. 2009 Dec 1;13(6):359-65.
Söderberg O, Gullberg M, Jarvius M, Ridderstråle K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, Landegren U. Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nat. Methods. 2006 Dec;3(12):995-1000.
Jarvius J, Melin J, Göransson J, Stenberg J, Fredriksson S, Gonzalez-Rey C, Bertilsson S, Nilsson M. Digital quantification using amplified single-molecule detection. Nat Methods. 2006 Sep;3(9):725-7.
Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wählby C, Gullberg M, Botling J, Sjöblom T, Markova B, Ostman A, Landegren U, Söderberg O. In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method. Mol. Cell Proteomics, 2007 Sep;6(9):1500-9.
Multiplex PLA: Fredriksson S, Dixon W, Ji H, Koong AC, Mindrinos M, Davis RW: Multiplexed protein detection by proximity ligation for cancer biomarker validation. Nat Methods 2007, 4(4):327-329. Söderberg O, Leuchowius KJ, Kamali-Moghaddam M, Jarvius M, Gustafsdottir S, Schallmeiner E, Gullberg M, Jarvius J, Landegren U: Proximity ligation: a specific and versatile tool for the proteomic era. Genet Eng (N Y) 2007, 28:85-93.
In solution PLA for pathogen detection: Gustafsdottir SM, Nordengrahn A, Fredriksson S, Wallgren P, Rivera E, Schallmeiner E, Merza M, Landegren U: Detection of individual microbial pathogens by proximity ligation Clin Chem 2006, 52(6):1152-1160
Gustafsdottir SM, Schallmeiner E, Fredriksson S, Gullberg M, Soderberg O, Jarvius M, Jarvius J, Howell M, Landegren U: Proximity ligation assays for sensitive and specific protein analyses Anal Biochem, 2005, 345(1):2-9
Zhu, L, Koistinen, H, Wu, P, Narvanen, A, Schallmeiner, E, Fredriksson, S, Landegren, U & Stenman, U H, A sensitive proximity ligation assay for active PSA Biol Chem, 2006, 387, 769-72
In solution PLA using antibodies: Gullberg, M, Gustafsdottir, S M, Schallmeiner, E, Jarvius, J, Bjarnegard, M, Betsholtz, C, Landegren, U & Fredriksson, S, Cytokine detection by antibody-based proximity ligation Proc Natl Acad Sci U S A, 2004, 101, 8420-4
Bjarnegard, M, Enge, M, Norlin, J, Gustafsdottir, S, Fredriksson, S, Abramsson, A, Takemoto, M, Gustafsson, E, Fassler, R & Betsholtz, C, Endothelium-specific ablation of PDGFB leads to pericyte loss and glomerular, cardiac and placental abnormalities Development, 2004, 131, 1847-57
Landegren, U, Nilsson, M, Gullberg, M, Soderberg, O, Jarvius, M, Larsson, C & Jarvius, J, Prospects for in situ analyses of individual and complexes of DNA, RNA, and protein molecules with padlock and proximity probes Methods Cell Biol, 2004, 75, 787-97 Lindblom, P, Gerhardt, H, Liebner, S, Abramsson, A, Enge, M, Hellstrom, M, Backstrom, G, Fredriksson, S, Landegren, U, Nystrom, H C, Bergstrom, G, Dejana, E, Ostman, A, Lindahl, P & Betsholtz, C, Endothelial PDGF-B retention is required for proper investment of pericytes in the microvessel wall Genes Dev, 2003, 17, 1835-40 Rollman, O, Jensen, U B, Ostman, A, Bolund, L, Gustafsdottir, S M & Jensen, T G, Platelet derived growth factor (PDGF) responsive epidermis formed from human keratinocytes transduced with the PDGF beta receptor gene J Invest Dermatol, 2003, 120, 742-9
In solution PLA using aptamers: Fredriksson, S, Gullberg, M, Jarvius, J, Olsson, C, Pietras, K, Gustafsdottir, S M, Ostman, A & Landegren, U, Protein detection using proximity-dependent DNA ligation assays Nat Biotechnol, 2002, 20, 473-7
DNA-Protein interactions: Gustafsdottir SM, Schlingemann J, Rada-Iglesias A, Schallmeiner E, Kamali-Moghaddam M, Wadelius C, Landegren U: In vitro analysis of DNA-protein interactions by proximity ligation. Proc Natl Acad Sci U S A 2007, 104(9):3067-3072. Schallmeiner E, Oksanen E, Ericsson O, Spangberg L, Eriksson S, Stenman UH, Pettersson K, Landegren U: Sensitive protein detection via triple-binder proximity ligation assays. Nat Methods 2007, 4(2):135-137.
Selector Technology
Stenberg, J, Dahl, F, Landegren, U & Nilsson, M, PieceMaker: selection of DNA fragments for selector-guided multiplex amplification Nucleic Acids Res, 2005, 33, e72
Dahl, F, Stenberg, J, Fredriksson, S, Welch, K, Zhang, M, Nilsson, M, Bicknell, D, Bodmer, WF, Davis, RW & Ji, H Multigene amplification and massively parallel sequencing for cancer mutation discovery Proc Natl Acad Sci USA 104 , 9387-9392 (2007)
Isaksson, M, Stenberg, J, Dahl, F, Thuresson, A-C, Bondeson, M-L & Nilsson, M MLGA - a rapid and cost-efficient assay for gene copy-number analysis Nucleic Acids Res 35 , e115 (2007)
Salmon Hillbertz, NHC, Isaksson, M, Karlsson, EK, Hellmen, E, Rosengren Pilgren, G, Savolainen, P, Wade, CM, von Euler, H, Gustafson, U, Hedhammar, Nilsson, M, Lindblad-Toh, K, Andersson, L & Andersson, G A duplication of FGF3, FGF4, FGF9 and ORAOV1 causes the hair ridge and predisposes to dermoid sinus in Ridgeback dogs Nat Genet 39 , 1318-1320 (2007)
ProbeMaker Software for oligonucleotide design: Stenberg, J, Nilsson, M & Landegren, U, ProbeMaker: an extensible framework for design of sets of oligonucleotide probes BMC Bioinformatics, 2005, 6:229
DNA amplification and detection
Jarvius, J, Melin, J, Goransson, J, Stenberg, J, Fredriksson, S, Gonzalez-Rey, C, Bertilsson, S & Nilsson, M, Digital quantification using amplified single-molecule detection Nat Methods, 2006, 3, 725-7
Melin, J, Jarvius, J, Göransson, J & Nilsson, M Homogenous amplified single-molecule detection - Characterization of key parameters Anal Biochem 368 , 230-238 (2007)
Blab, G A, Schmidt, T & Nilsson, M, Homogeneous detection of single rolling circle replication products Anal Chem, 2004, 76, 495-8
Circle-to-Circle amplification: Dahl, F, Baner, J, Gullberg, M, Mendel-Hartvig, M, Landegren, U & Nilsson, M, Circle-to-circle amplification for precise and sensitive DNA analysis Proc Natl Acad Sci U S A, 2004, 101, 4548-53
Padlock Probe Technology
Nilsson, M., Dahl, F., Larsson, C., Gullberg, M. & Stenberg, J., Analyzing genes using closing and replicating circles. Trends Biotechnol, 2006, 24, 83-8. In situ Genotyping: Inversion probes: Padlock Probes on RNA: Padlock Probes and Rolling Circle Amplification: Original Paper on Padlock Probes: Nilsson, M., Malmgren, H., Samiotaki, M., Kwiatkowski, M., Chowdhary, B. P. & Landegren, U., Padlock probes: circularizing oligonucleotides for localized DNA detection. Science, 1994, 265, 2085-8.